AKT2 Antibody

Instruction Manual!

Product name:	AKT2 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC270769

Product Name	AKT2 Antibody
Antibody Type	Primary Antibodies
Productdescription	AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.1) Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003). Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003). Jones, D.T., et al., Blood 101(8):3174-3180 (2003). Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003). More
Immunogen	This AKT2 antibody is generated from rabbits immunized with human AKT2 recombinant protein.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFWB
	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity	HumanMouse
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	AKT2
Alternative Names	RAC-beta serine/threonine-protein kinase Protein kinase Akt-2 Protein kinase B beta PKB beta RAC-PK-beta AKT2 More
Molecular Weight(MW)	56 kDa

Entrez Gene	208
Protein Accession	P31751

Application

Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35ug/lane)
Application

Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35ug/lane)
Application

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

ApplicationNotes	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Form	Liquid
Storagenstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.