MEK1 / 2 (phospho Ser218 / Ser222) Antibody

Instruction Manual!

Product name:	MEK1 / 2 (phospho Ser218 / Ser222) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:	WB
Storage:	For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
UOM:	100ug

MEK1 / 2 (phospho Ser218 / Ser222) Antibody

Catalog Number：IC284536

Product Profile

Product Name	MEK1 / 2 (phospho Ser218 / Ser222) Antibody
Antibody Type	Primary Antibodies
Productdescription	MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade.1) N.G. Ahn, N.G., Robbins, D.G., Haycock, J.W., Seger, R., Cobb, M.H. and Krebs, E.G., "Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin," J. Neurochem. 59 (1992) 147 - 156. More
Immunogen	MEK 1/2 (Ser218/222) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer218/222 of human MEK 1/2.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
TestedApplications	WB
	The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: More
Species Reactivity	HumanMouseRatXenopus
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Gene Symbol	MAP2K1
Alternative Names	CFC3 MEK1 MKK1 MAPKK1 PRKMK1 ERK activator kinase 1 MAP kinase kinase 1 More
Molecular Weight(MW)	45
Tissue Specificity	MEK1/2 antibody is specific for MEK 1/2 phosphorylated at Ser218/222.

Database Links

Entrez Gene	5604
Protein Accession	Q02750

Application

Application

Western blot of NIH 3T3 cell lysates. The cells were serum starved (control) or incubated in the presence of serum (serum) for 5 minutes. The western blot shows the immunolabeling of the ~45k MEK1/2 was absent in control and stimulated by serum.

ApplicationNotes	The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: More

ApplicationNotes

The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:

Form	Liquid
StorageInstructions	For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Storage Buffer	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

MEK1 / 2 (phospho Ser218 / Ser222) Antibody

MEK1 / 2 (phospho Ser218 / Ser222) Antibody

Product Profile

Key Feature

Target Information

Database Links

Application

Application

Additional Information