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MEK1 (phospho Thr386) Antibody

MEK1 (phospho Thr386) Antibody MEK1 (phospho Thr386) Antibody

Instruction Manual!

Product name: MEK1 (phospho Thr386) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:
Storage:For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
UOM:100ug

MEK1 (phospho Thr386) Antibody

Catalog Number:IC284535

Product Profile

Product Name MEK1 (phospho Thr386) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.1) Park, S.H., Zarrinpar, A. and Lim, W.A., "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms," Science 299 (2003) 1061 - 1064.
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Immunogen
MEK 1 (Thr386) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr386 of human MEK 1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol MAP2K1
Alternative Names
CFC3
MEK1
MKK1
MAPKK1
PRKMK1
ERK activator kinase 1
MAP kinase kinase 1
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Molecular Weight(MW) 45
Tissue Specificity MEK1 antibody is specific for the 45k MEK 1 phosphorylated at Thr386.

Database Links

Entrez Gene 5604
Protein Accession Q02750

Application

  • Application

    Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr386.

ApplicationNotes
 Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
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Additional Information

Form Liquid
StorageInstructions  For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Storage Buffer 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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