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Mouse Map2k1 Antibody

Mouse Map2k1 Antibody Mouse Map2k1 Antibody

Instruction Manual!

Product name: Mouse Map2k1 Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:IHC-PWB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

Mouse Map2k1 Antibody

Catalog Number:IC285144

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Product Profile

Product Name Mouse Map2k1 Antibody
Antibody Type Primary Antibodies
Productdescription 
							
							
							
Map2k1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
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Immunogen
This Mouse Map2k1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-296 amino acids from the C-terminal region of mouse Map2k1.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB

For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol Map2k1
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 1
MAP kinase kinase 1
MAPKK 1
ERK activator kinase 1
MAPK/ERK kinase 1
MEK 1
Map2k1
Mek1
Prkmk1
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Molecular Weight(MW) 43 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Hamster

Database Links

Entrez Gene 26395
Protein Accession P31938

Application

  • Application

    Mouse Map2k1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Application

    Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).

  • Application

    Western blot analysis of Map2k1 using rabbit polyclonal Mouse Map2k1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k1 gene.

  • Application

    Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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