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MEK1/2 Antibody

MEK1/2 Antibody MEK1/2 Antibody

Instruction Manual!

Product name: MEK1/2 Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:MEK1/2 antibody is supplied in PBS containing 0.02% sodium azide.
Applications:ELISAIFIHC-PWB
Storage:MEK1/2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
UOM:100ug

MEK1/2 Antibody

Catalog Number:IC284540

Product Profile

Product Name MEK1/2 Antibody
Antibody Type Primary Antibodies
Productdescription
							
The mitogen-activated protein kinase kinase 1 (MAP2K1, also known as MEK1) and the highly homologous MEK2 are members of the dual specificity protein kinase family (1). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals (2). MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (2).1) Zheng CF and Guan KL. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 1993; 268:11435-9.
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Immunogen
MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1. The immunogen is located within amino acids 240 - 290 of MEK1/2.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
TestedApplications 

ELISAIFIHC-PWB

MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:
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Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol MAP2K1
Alternative Names
Mitogen-activated protein kinase kinase 1
MAP2K1
MAPKK1
MEK1
MKK1
PRKMK1
CFC3
Mitogen-activated protein kinase kinase 2
MAP2K2
MAPKK2
MEK2
MKK2
PRKMK2
CFC
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MolecularWeight(MW)  Predicted: 43 kDa Observed: 44 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Rabbit: (100%)
Tissue Specificity MEK1/2 antibody is human, mouse, and rat reactive. This antibody detects both MEK1 and MEK2.

Database Links

Entrez Gene 5604
Protein Accession NP_002746

Application

  • Application

    Immunofluorescence of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 20 μg/mL.

  • Application

    Immunohistochemistry of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 5 μg/mL.

Positive Control 1) Cat. No. 1303 - Human Brain Tissue Lysate
ApplicationNotes
MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:
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Additional Information

Form Liquid
StorageInstructions  MEK1/2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Storage Buffer MEK1/2 antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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