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MEK1 (phospho Ser221) Antibody
Instruction Manual!
| Product name: | MEK1 (phospho Ser221) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IFIHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
MEK1 (phospho Ser221) Antibody
Catalog Number：IC284533
Product Profile
| Product Name | MEK1 (phospho Ser221) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
MEK1 (Phospho-Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 221 (A-N-S (p) -F-V) derived from Human MEK1. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | MAP2K1 |
|---|---|
| Alternative Names |
CFC3
More
MEK1 MKK1 MAPKK1 PRKMK1 EMS1 ERK activator kinase 1 MAP kinase kinase 1 MAP2K1 MAPK/ERK kinase 1 MAPKK 1 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of MEK1 only when phosphorylated at serine 221. |
Database Links
| Entrez Gene | 5604 |
|---|---|
| Protein Accession | NP_002746.1 |
Application
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK1 (Phospho-Ser221).
-
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MEK1 (Phospho-Ser221).
| Application Notes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
