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MEK1 / MEK2 Antibody

MEK1 / MEK2 Antibody MEK1 / MEK2 Antibody

Instruction Manual!

Product name: MEK1 / MEK2 Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IFIHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

MEK1 / MEK2 Antibody

Catalog Number:IC284538

Product Profile

Product Name MEK1 / MEK2 Antibody
Antibody Type Primary Antibodies
Productdescription
							
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Shen R, et al. (2002) Mol Cell Biol; 22 (10): 3230-3236Preisinger C, et al. (2005) EMBO J; 24 (4): 753-765Laine P, et al. (2000) Biochem J; 349 (Pt 1): 19-25Yaglom J, et al. (2003) Mol Cell Biol; 23 (11): 3813-3824Dahan S, et al. (2002) Infect Immun; 70 (5): 2304-2310
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Immunogen
MEK1/MEK2 (Ab-217/221) antibody was raised against a peptide sequence around aa.215~219/219~223 (I-D-S-M-A) derived from Human MEK1/MEK2.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications

 IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol MAP2K1 MAP2K2
Alternative Names
CFC3
MEK1
MKK1
MAPKK1
PRKMK1
GLUR2
ERK activator kinase 1/ERK activator kinase2
MAP kinase kinase 1/MAP kinase kinase 2
MAP2K1/MAP2K1
MAPK/ERK kinase 1/MAPK/ERK kinase 2
MAPKK 1/MAPKK 1
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Molecular Weight(MW) 45 kDa
Tissue Specificity This antibody detects endogenous level of total MEK1/MEK2 protein.

Database Links

Entrez Gene 5604 / 5605
Protein Accession NP_002746.1 / NP_109587.1

Application

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MEK1/MEK2 (Ab-217/221). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK1/MEK2(Ab-217/221).

  • Application

    Western blot analysis of lysed extracts from 293 cells using MEK1/MEK2 (Ab-217/221).

ApplicationNotes 
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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