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syk (phospho Tyr323) Antibody

syk (phospho Tyr323) Antibody syk (phospho Tyr323) Antibody

Instruction Manual!

Product name: syk (phospho Tyr323) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IFIHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

syk (phospho Tyr323) Antibody

Catalog Number:IC292609

Product Profile

Product Name syk (phospho Tyr323) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C γdependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion1) Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447. Turner, M. et al. (2000) Immunol. Today 21, 148-154. Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
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Immunogen
syk (phospho-Tyr323) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y (p) -E- P) derived from Human syk.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol SYK
Alternative Names
p72-Syk
HSP27
HSP28
Spleen tyrosine kinase
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Molecular Weight(MW) 72 kDa
Tissue Specificity This antibody detects endogenous level of syk only when phosphorylated at tyrosine 323.

Database Links

Entrez Gene 6850
Protein Accession NP_001128524.1

Application

  • Application

    Western blot analysis of lysed extracts from JK cells untreated or treated with H2O2 for the indicated times, using syk (phospho-Tyr323).

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk (Phospho-Tyr323). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using syk (phospho-Tyr323).

ApplicationNotes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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