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p27Kip1 (phospho Thr187) Antibody

p27Kip1 (phospho Thr187) Antibody p27Kip1 (phospho Thr187) Antibody

Instruction Manual!

Product name: p27Kip1 (phospho Thr187) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

p27Kip1 (phospho Thr187) Antibody

Catalog Number:IC287142

Product Profile

Product Name p27Kip1 (phospho Thr187) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.1) Eguchi H, et al. (2003) Cancer Res; 63 (15): 4739-46Le XF, et al. (2003) J Biol Chem;Connor MK, et al. (2003) ) Mol Biol Cell; 14 (1): 201-13Fujita N, et al. (2002) J Biol Chem; 277 (32): 28706-13Ishida N, et al. (2000) J Biol Chem; 275 (33): 25146-54
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Immunogen
p27Kip1 (Phospho-Thr187) antibody was raised against a peptide sequence around phosphorylation site of threonine 187 (E-Q-T (p) -P-K) derived from Human p27Kip1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CDKN1B
Alternative Names
KIP1
MEN4
CDKN4
MEN1B
P27KIP1
MAPTL
MTBT1
TAU
CDKN1B
CDN1B
Cyclin-dependent kinase inhibitor 1B
Cyclin-dependent kinase inhibitor p27
KIP1
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MolecularWeight(MW)  27 kDa
Tissue Specificity This antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.

Database Links

Entrez Gene 1027
Protein Accession NP_004055.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187).

  • Application

    Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, IFN-α using p27Kip1 (Phospho-Thr187).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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