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Caspase 9 (phospho Thr125) Antibody

Caspase 9 (phospho Thr125) Antibody Caspase 9 (phospho Thr125) Antibody

Instruction Manual!

Product name: Caspase 9 (phospho Thr125) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Caspase 9 (phospho Thr125) Antibody

Catalog Number:IC273482

Product Profile

Product Name Caspase 9 (phospho Thr125) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
							
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.1) Duan H., J. Biol. Chem. 271:16720-16724(1996).
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Immunogen
Caspase 9 (phospho Thr125) antibody was raised against a peptide sequence around phosphorylation site of threonine 125 (P-E-T (p)-P-R) derived from Human Caspase 9.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CASP9
Alternative Names
APAF-3
MCH6
RNCASP9
CASP-9
ICE-LAP6
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MolecularWeight(MW)  47 kDa
Tissue Specificity The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).

Database Links

Entrez Gene 842
Protein Accession NP_001220.2

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Application

    Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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