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CDC2 (phospho Thr161) Antibody

CDC2 (phospho Thr161) Antibody CDC2 (phospho Thr161) Antibody

Instruction Manual!

Product name: CDC2 (phospho Thr161) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

CDC2 (phospho Thr161) Antibody

Catalog Number:IC275034

Product Profile

Product Name CDC2 (phospho Thr161) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.1) Ukomadu C, et al. (2003) J Biol Chem; 278 (7): 4840-6. Morris MC, et al. (2002) J Biol Chem; 277 (26): 23847-53. Brown NR, et al. (1999) J Biol Chem; 274 (13): 8746-56. Liu Y, et al. (2004) J Biol Chem; 279 (6): 4507-14.
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Immunogen
CDC2 (Phospho-Thr161) antibody was raised against a peptide sequence around phosphorylation site of threonine161 (T-Y-T (p) -H-E) derived from Human CDC2.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CDK1
Alternative Names
CDC2
CDC28A
P34CDC2
C1orf215
LAP18
OP18
CDC28
CDC2A
CDK1
Cyclin-dependent kinase 1
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Molecular Weight(MW) 34 kDa
Tissue Specificity This antibody detects endogenous level of CDC2 only when phosphorylated at threonine 161.

Database Links

Entrez Gene 983
Protein Accession NP_001163877.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 (Phospho-Thr161).

  • Application

    Western blot analysis of lysed extracts from HeLa cells untreated (Lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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