TrkA-pY791 Antibody

Instruction Manual!

Product name:	TrkA-pY791 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC294146

Product Name	TrkA-pY791 Antibody
Antibody Type	Primary Antibodies
Productdescription	TRKA (also known as NTRK1)is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer.1) Tokusashi, Y., et al., Int. J. Cancer 114(1):39-45 (2005). More
Immunogen	This TrkA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 769-796 amino acids from human TrkA.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIHC-PWB
	For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000:
Species Reactivity	HumanMouse
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	NTRK1
Alternative Names	High affinity nerve growth factor receptor Neurotrophic tyrosine kinase receptor type 1 TRK1-transforming tyrosine kinase protein Tropomyosin-related kinase A Tyrosine kinase receptor Tyrosine kinase receptor A Trk-A gp140trk p140-TrkA NTRK1 MTC More
Molecular Weight(MW)	87 kDa

Entrez Gene	4914
Protein Accession	P04629

Application

Overlay histogram showing SH-SY5Y cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(O
Application

Antibody staining TrkA in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application

Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.
Application

Western blot analysis of hTrkA-pY791 in HepG2 cell line lysates (35ug/lane)
Application

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

TrkA-pY791 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

ApplicationNotes	For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.