TrkA Antibody

Instruction Manual!

Product name:	TrkA Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC294145

Product Name	TrkA Antibody
Antibody Type	Primary Antibodies
Productdescription	TRKA (also known as NTRK1)is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer.1) Tokusashi, Y., et al., Int. J. Cancer 114(1):39-45 (2005). More
Immunogen	This TrkA antibody is generated from rabbits immunized with TrkA his fusion protein

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFWB
	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity	HumanMouse
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	NTRK1
Alternative Names	High affinity nerve growth factor receptor Neurotrophic tyrosine kinase receptor type 1 TRK1-transforming tyrosine kinase protein Tropomyosin-related kinase A Tyrosine kinase receptor Tyrosine kinase receptor A Trk-A gp140trk p140-TrkA NTRK1 MTC More
Molecular Weight(MW)	87 kDa

Entrez Gene	4914
Protein Accession	P04629

Application

Western blot analysis of TrkA Antibody in mouse brain tissue lysates (35ug/lane)
Application

Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Confocal immunofluorescent analysis of TrkA Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

ApplicationNotes	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.