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c ABL Antibody

c ABL Antibody c ABL Antibody

Instruction Manual!

Product name: c ABL Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:IHC-PWB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

c ABL Antibody

Catalog Number:IC272818

Product Profile

Product Name c ABL Antibody
Antibody Type Primary Antibodies
Productdescription
							
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.1) Donaldson, L.W., et al., Proc. Natl. Acad. Sci. U.S.A. 99(22):14053-14058 (2002).
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Immunogen
This c ABL (ABL1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 851-881 amino acids from the C-terminal region of human c ABL (ABL1).

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB

For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol ABL1
Alternative Names
Tyrosine-protein kinase ABL1
Abelson murine leukemia viral oncogene homolog 1
Abelson tyrosine-protein kinase 1
Proto-oncogene c-Abl
p150
ABL1
ABL
JTK7
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Molecular Weight(MW) 123 kDa

Database Links

Entrez Gene 25
Protein Accession P00519

Application

  • Application

    Western blot analysis in MCF-7 cell line lysates (35ug/lane).

  • Application

    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Application Notes
For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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