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HSF1 Sumoylation Site Antibody

HSF1 Sumoylation Site Antibody HSF1 Sumoylation Site Antibody

Instruction Manual!

Product name: HSF1 Sumoylation Site Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:IFWB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

HSF1 Sumoylation Site Antibody

Catalog Number:IC281364

Product Profile

Product Name HSF1 Sumoylation Site Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.1) Hilgarth, et al., Biochem Biophys Res Commun. 2003 Mar 28;303(1):196-200.
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Immunogen
This HSF1 Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-309 amino acids from human HSF1 Sumoylation Site.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFWB

For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Unpurified

Target Information

Gene Symbol HSF1
Alternative Names
Heat shock factor protein 1
HSF 1
Heat shock transcription factor 1
HSTF 1
HSF1
HSTF1
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Molecular Weight(MW) 57 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine

Database Links

Entrez Gene 3297
Protein Accession Q00613

Application

  • Application

    Western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).

  • Application

    Confocal immunofluorescent analysis of HSF1 Sumoylation Site Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Application Notes
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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