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PYK2 (phospho Tyr579) Antibody

PYK2 (phospho Tyr579) Antibody PYK2 (phospho Tyr579) Antibody

Instruction Manual!

Product name: PYK2 (phospho Tyr579) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

PYK2 (phospho Tyr579) Antibody

Catalog Number:IC289330

Product Profile

Product Name PYK2 (phospho Tyr579) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.1) Lev S., Nature 376:737-745(1995).
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Immunogen
PYK2 (phospho Tyr579) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 579 (E-D-Y (p)-Y-K) derived from Human PYK2.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol PTK2B
Alternative Names
CADTK
FADK 2
FAK2
PTK2B
RAFTK
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MolecularWeight(MW)  116 kDa
Tissue Specificity The antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.

Database Links

Entrez Gene 2185
Protein Accession NP_004094.3

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Application

    Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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