Androgen Receptor Antibody

Instruction Manual!

Product name:	Androgen Receptor Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	IHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC271057

Product Name	Androgen Receptor Antibody
Antibody Type	Primary Antibodies
Productdescription	Androgen receptor (ANDR) has 3 major functional domains: the N-terminal domain, DNA-binding domain, and an androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The gene for this protein contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of the protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations are also associated with complete androgen insensitivity (CAIS). PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor; sumoylation of ANDR represses androgen receptor dependent transcription.1) Nishida, et al. J. Biol. Chem. 277 (44), 41311-41317 (2002) More
Immunogen	This Androgen Receptor (ANDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-392 amino acids from human Androgen Receptor (ANDR).

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity	HumanMouse
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	AR
Alternative Names	Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 More
Molecular Weight(MW)	99 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Monkey

Entrez Gene	367
Protein Accession	P10275

Application

Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the AR antibody detected the AR protein (arrow).
Application

Western blot analysis in Hela cell line lysates (35ug/lane).This demondetected the ANDR protein (arrow).
Application

Androgen Receptor Antibody (ANDR) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Androgen Receptor Antibody (ANDR) for immunohistochemistry.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.