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CCND1-Y226 Antibody

CCND1-Y226 Antibody CCND1-Y226 Antibody

Instruction Manual!

Product name: CCND1-Y226 Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:IFWB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

CCND1-Y226 Antibody

Catalog Number:IC273794

Product Profile

Product Name CCND1-Y226 Antibody
Antibody Type Primary Antibodies
Productdescription 
							
CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.1) Edel, M.J., et al. Genes Dev. 24(6):561-573(2010)
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Immunogen
This CCND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from human CCND1.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFWB

For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CCND1
Alternative Names
G1/S-specific cyclin-D1
B-cell lymphoma 1 protein
BCL-1
BCL-1 oncogene
PRAD1 oncogene
CCND1
BCL1
PRAD1
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Molecular Weight(MW) 34 kDa

Database Links

Entrez Gene 595
Protein Accession P24385

Application

  • Application

    Confocal immunofluorescent analysis of CCND1-Y226 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • Application

    Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstratesdetected the CCND1 protein (arrow).

Application Notes
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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