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Cyclin D1 (phospho Thr286) Antibody

Cyclin D1 (phospho Thr286) Antibody Cyclin D1 (phospho Thr286) Antibody

Instruction Manual!

Product name: Cyclin D1 (phospho Thr286) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Cyclin D1 (phospho Thr286) Antibody

Catalog Number:IC276529

Product Profile

Product Name Cyclin D1 (phospho Thr286) Antibody
Antibody Type Primary Antibodies
Productdescription
							
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.1) Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40
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Immunogen
Cyclin D1 (phospho Thr286) antibody was raised against a peptide sequence around phosphorylation site of threonine 286 (A-C-T (p)-P-T) derived from Human Cyclin D1.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Species Reactivity

MouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CCND1
Alternative Names
BCL-1 
oncogene
CYL-1
PRAD1
PRAD1
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MolecularWeight(MW)  33 kDa
Tissue Specificity The antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286.

Database Links

Entrez Gene 595
Protein Accession NP_444284.1

Application

  • Application

    Western blot analysis of extracts from MCF7 tissue using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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