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RSK1 Antibody

RSK1 Antibody RSK1 Antibody

Instruction Manual!

Product name: RSK1 Antibody
Source:Mouse
Purity:>95%
Buffer Formulation:Ascitic fluid containing 0.03% sodium azide.
Applications:ELISAIHCWB
Storage:RSK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

RSK1 Antibody

Catalog Number:IC290407

Product Profile

Product Name RSK1 Antibody
Antibody Type Primary Antibodies
Productdescription
							
Rsk1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine / threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK proteins possess two separate kinase domains, the C terminal kinase domain and the N terminal kinase domain, separated by a linker region containing a hydrophobic motif. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation.1) Alcorta, D.A., et al. 1989. Mol. Cell. Biol. 9:3850-3859.
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Immunogen
Ni-NTA purified truncated recombinant RSK1 expressed in E. Coli strain BL21 (DE3).

Key Feature

Clonality Monoclonal
Isotype IgG2a
Host Species Mouse
TestedApplications 

ELISAIHCWB

Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Species Reactivity

Human

Concentration 1mg/ml
Purification

Target Information

Gene Symbol RPS6KA1
Alternative Names
MAPKAPK1A
RSK1
90 kDa ribosomal protein S6 kinase 1
S6K-alpha-1
RSK
HU-1
MAPKAPK1A
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Database Links

Entrez Gene 6195
Protein Accession Q15418

Application

  • Application

    Western blot analysis using RSK1 monoclonal antibody against truncated RSK1 - His recombinant protein.

  • Application

    Immunohistochemical analysis of paraffin - embedded human colon adenocarcinoma (top) and human liver carcinoma (bottom) showing nuclear location using RSK1 antibody with DAB staining.

ApplicationNotes
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:

Additional Information

Form Liquid
StorageInstructions RSK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Ascitic fluid containing 0.03% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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