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IRF3 antibody - C-terminal region
Instruction Manual!
| Product name: | IRF3 antibody - C-terminal region |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-IRF3 polyclonal antibody - C-terminal region
Catalog Number：IC108557
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Product Profile
| ProductName | Rabbit anti-IRF3 polyclonal antibody - C-terminal region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-IRF3 antibody: synthetic peptide directed towards the C terminal of human IRF3 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Protein A |
Target Information
| Gene Symbol | IRF3 |
|---|---|
| Gene Synonyms |
NR4A1
|
| Gene Full Name | Interferon regulatory factor 3 |
| Gene Summary |
IRF3 is interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
More
|
| Alternative Names |
HMR
More
N10 TR3 NP10 GFRP1 NAK-1 NGFIB NUR77 |
| MolecularWeight(MW) | 47kDa |
| Sequence | 427 amino acids |
Database Links
| Entrez Gene | 3661 |
|---|---|
| SwissProt ID | Q14653 |
| Protein Accession | NP_001562 |
Application
-
Application
Host: Rabbit
Target Name: NR4A1
Sample Tissue: Human Hela
Antibody Dilution: 1.0ug/ml -
Application
Rabbit Anti-NR4A1 Antibody
Catalog Number: ARP31941
Paraffin Embedded Tissue: Human Stomach
Cellular Data: Mucous Cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X -
Application
Rabbit Anti-NR4A1 Antibody
Catalog Number: ARP31941
Paraffin Embedded Tissue: Human Liver
Cellular Data: Hepatocytes
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X -
Application
Lanes:
Lane 1: 50ug mouse embryonic fibroblast lysate
Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate
Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Donkey anti-Rabbit-HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
PTRF
Submitted by:
Libin Liu, Boston University. -
Application
Host: Rabbit
Target Name: PTRF
Sample Tissue: Mouse Lung
Antibody Dilution: 1.0ug/ml -
Application
IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.
-
Application
Lanes:
Lane 1: 5 ug 293T cell lysate
Lane 2: 10 ug 293T cell lysate
Lane 3: 20 ug 293T cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
HMGA2
Submitted by:
AnonymousHMGA2 is supported by BioGPS gene expression data to be expressed in HEK293T
-
Application
WB Suggested Anti-TRIM32 Antibody Titration:1.25ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate -
Application
PKLR antibody - N-terminal region (ARP41699_T100) validated by WB using HepG2 cell lysate at 1.0ug/ml.
There is BioGPS gene expression data showing that PKLR is expressed in HepG2
-
Application
Rabbit Anti-PKLR Antibody
Catalog Number: ARP41699
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X -
Application
Sample Type: Equine Cartilage Explants
Primary Dilution: 1:800
Secondary Antibody: Bio-Rad 170-5046
Secondary Dilution::100,000
Image Submitted By: Adam Williams
University of Nottingham -
Application
WB Suggested Anti-MMP1 Antibody
Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate
Primary Antibody Dilution : 1:500
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:2000
Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore -
Application
Host: Rabbit
Target Name: MMP1
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml -
Application
WB Suggested Anti-PCGF3 Antibody Titration:0.125ug/ml
ELISA Titer: 1:12500
Positive Control: Raji cell lysatePCGF3 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells
-
Application
Host: Rabbit
Target Name: PCGF3
Sample Type: Fetal Muscle lysates
Antibody Dilution: 1.0ug/ml -
Application
Host: Rabbit
Target Name: F13B
Sample Tissue: Human DLD1
Antibody Dilution: 1.0ug/ml -
Application
Rabbit Anti-F13B antibody
Catalog Number: ARP41999
Formalin Fixed Paraffin Embedded Tissue: Human Fetal Liver
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec -
Application
Rabbit Anti-F13B antibody
Catalog Number: ARP41999
Formalin Fixed Paraffin Embedded Tissue: Human Vessel
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec -
Application
WB Suggested Anti-FBXO25 Antibody Titration:0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human heart -
Application
Host: Rabbit
Target Name: MARCH5
Sample Type: U937 Whole Cell lysates
Antibody Dilution: 1.0ug/ml -
Application
WB Suggested Anti-SLC8A3 Antibody Titration:0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysate -
Application
WB Suggested Anti-SULT6B1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate -
Application
WB Suggested Anti-TMTC1 Antibody Titration:0.2-1 ug/ml
Positive Control: Human heart -
Application
WB Suggested Anti-ZNF599 Antibody Titration:0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysate -
Application
WB Suggested Anti-SSX4B Antibody Titration:0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human kidney -
Application
WB Suggested Anti-UBQLN1 Antibody Titration:1 ug/ml
Positive Control: 293T cells lysate -
Application
Immunohistochemistry with Human Lung, respiratory epethelium tissue at an antibody concentration of 5.0ug/ml using anti-UBQLN1 antibody (ARP54958_P050).
-
Application
Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. UBQLN1 Antibody ARP54958_P050 concentration 5 ug/ml.
-
Application
WB Suggested Anti-Vwa5a Antibody
Titration: 1.0 ug/ml
Positive Control: Mouse Kidney -
Application
Host: Rabbit
Target Name: SPATA2
Sample Type: HCT15 Whole Cell lysates
Antibody Dilution: 1.0ug/ml -
Application
WB Suggested Anti-ADAM8 Antibody Titration:0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: PANC1 cell lysate -
Application
WB Suggested Anti-ORMDL3 Antibody
Titration: 1.0 ug/ml
Positive Control: Jurkat Whole Cell -
Application
WB Suggested Anti-GLIS2 Antibody Titration:2.5ug/ml
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate -
Application
WB Suggested Anti-RBM14 Antibody Titration:1.25ug/ml
ELISA Titer: 1:312500
Positive Control: SP2/0 cell lysate -
Application
Lanes:
Lane 1: 50ug Hela Lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
RBM14
Submitted by:
Archa Fox, University of Western Australia -
Application
WB Suggested Anti-TRIM32 Antibody Titration:1.025 ug/ml
Positive Control: 293T cells lysateTRIM32 is supported by BioGPS gene expression data to be expressed in HEK293T
-
Application
Human Muscle
-
Application
Host: Rabbit
Target Name: MARCH5
Sample Type: Human Fetal Kidney
Antibody Dilution: 1.0ug/ml -
Application
Host: Rabbit
Target Name: G6pc
Sample Tissue: Mouse Lung
Antibody Dilution: 1.0ug/ml -
Application
Host: Rabbit
Target Name: G6PC
Sample Tissue: Mouse Testis
Antibody Dilution: 1ug/ml -
Application
Host: Rabbit
Target Name: SPPL2B
Sample Type: 293T Whole cell lysates
Antibody Dilution: 1.0ug/ml -
Application
WB Suggested Anti-TMTC1 Antibody Titration:0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Spleen -
Application
WB Suggested Anti-NUP35 Antibody Titration:0.2-1 ug/ml
Positive Control: Jurkat cell lysate -
Application
WB Suggested Anti-Amigo2 Antibody
Titration: 1.0 ug/ml
Positive Control: Rat Heart -
Application
WB Suggested Anti-ACO2 Antibody Titration:0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysateACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
-
Application
ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
