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p90 RSK (phospho Thr573) Antibody

p90 RSK (phospho Thr573) Antibody p90 RSK (phospho Thr573) Antibody

Instruction Manual!

Product name: p90 RSK (phospho Thr573) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

p90 RSK (phospho Thr573) Antibody

Catalog Number:IC287219

Product Profile

Product Name p90 RSK (phospho Thr573) Antibody
Antibody Type Primary Antibodies
Productdescription
							
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes.1) Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206.
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Immunogen
p90 RSK (phospho Thr573) antibody was raised against a peptide sequence around phosphorylation site of threonine 573 (L-M-T (p)-P-C) derived from Human p90 RSK.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol RPS6KA1
Alternative Names
KS6A1
p90RSK1
RSK1
RPS6KA1
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MolecularWeight(MW)  95 kDa
Tissue Specificity The antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 573.

Database Links

Entrez Gene 6195
Protein Accession NP_002944.2

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Application

    Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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