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GJA1 antibody - N-terminal region
Instruction Manual!
| Product name: | GJA1 antibody - N-terminal region |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-GJA1 polyclonal antibody - N-terminal region
Catalog Number：IC107898
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Product Profile
| Product Name | Rabbit anti-GJA1 polyclonal antibody - N-terminal region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-GJA1 antibody: synthetic peptide directed towards the N terminal of human GJA1 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | GJA1 |
|---|---|
| Gene Synonyms |
HSS
More
CX43 GJAL ODDD AVSD3 HLHS1 DFNB38 |
| Gene Full Name | Gap junction protein, alpha 1, 43kDa |
| Gene Summary |
Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
More
|
| Alternative Names |
HSS
More
CX43 GJAL ODDD AVSD3 HLHS1 DFNB38 |
| MolecularWeight(MW) | 42kDa |
| Sequence | 382 amino acids |
Database Links
| Entrez Gene | 2697 |
|---|---|
| SwissProt ID | Q53FJ6 |
| Protein Accession | NP_000156 |
Application
-
Western blot
Positive Control: Lane1: 60ug rat stiatum
Primary Antibody Dilution : 1:1000
Secondary Antibody : Goat anti-rabbit-IRDye800
Secondry Antibody Dilution : 1:10,000
Submitted by: Ruben van Vugt, The Nijmegen Centre for Molecular Life Sciences (NCMLS) -
Immunoblot
Lane1: 8 ug total cardiac lysate
Lane2: 15 ug total cardiac lysate
Lane3: 30 ug total cardiac lysate
Lane4: 50 ug total cardiac lysate
Primary Antibody Dilution:
0.25 ug/ml
Secondary Antibody:
Secondary Antibody Dilution:
Gene Name:
GJA1
Submitted by:
Anonymous -
Western blot
Species+tissue/cell type: Total rat cardiac lysate
1: 8 ug total cardiac lysate
2: 15 ug total cardiac lysate
3: 30 ug total cardiac lysate
4: 50 ug total cardiac lysatePrimary antibody dilution: 0.25 ug/ml
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
