Connexin 43 Antibody

Instruction Manual!

Product name:	Connexin 43 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	WB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC275921

Product Name	Connexin 43 Antibody
Antibody Type	Primary Antibodies
Productdescription	Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.1) Spinella, F., et al., J. Biol. Chem. 278(42):41294-41301 (2003). Contreras, J.E., et al., Proc. Natl. Acad. Sci. U.S.A. 100(20):11388-11393 (2003). Qin, H., et al., J. Biol. Chem. 278(32):30005-30014 (2003). Cameron, S.J., et al., J. Biol. Chem. 278(20):18682-18688 (2003). Ma, X.D., et al., World J. Gastroenterol. 9(5):946-950 (2003). More
Immunogen	This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	WB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein G

Gene Symbol	GJA1
Alternative Names	Gap junction alpha-1 protein Connexin-43 Cx43 Gap junction 43 kDa heart protein GJA1 GJAL More
Molecular Weight(MW)	43 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Xenopus

Entrez Gene	2697
Protein Accession	P17302

Application

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application

(LEFT)Western blot analysis of anti-GJA1 Antibody (N-term) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of hGJA1-N121.Connexin in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab.
Application

Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121) at 1:1000 at each lane.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.