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Connexin Antibody

Connexin Antibody Connexin Antibody

Instruction Manual!

Product name: Connexin Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:WB
Storage:Connexin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
UOM:100ug

Connexin Antibody

Catalog Number:IC275924

Product Profile

Product Name Connexin Antibody
Antibody Type Primary Antibodies
Productdescription 
							
							
Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).1) Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
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Immunogen
Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

WB

The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
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Species Reactivity

ChickenDogHumanMouseRatSheepXenopusZebra Fish

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol Gja1
Alternative Names
Cx43
Cxn-43
Connexin-43
Cx43
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Molecular Weight(MW) 43

Database Links

Entrez Gene 24392
Protein Accession P08050

Application

  • Application

    Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.

ApplicationNotes
The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
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Additional Information

Form Liquid
Storage Instructions Connexin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Storage Buffer 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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