MAP2K2 Antibody

Instruction Manual!

Product name:	MAP2K2 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	WB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：Ic284106

Product Name	MAP2K2 Antibody
Antibody Type	Primary Antibodies
Productdescription	MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.1) Burroughs, K.D., et al., Mol. Cancer Res. 1(4):312-322 (2003). More
Immunogen	This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from human MAP2K2.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	MAP2K2
Alternative Names	Dual specificity mitogen-activated protein kinase kinase 2 MAP kinase kinase 2 MAPKK 2 ERK activator kinase 2 MAPK/ERK kinase 2 MEK 2 MAP2K2 MEK2 MKK2 PRKMK2 More
Molecular Weight(MW)	44 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Drosophila

Entrez Gene	5605
Protein Accession	P36507

Application

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35ug/lane)
Application

Western blot analysis of MAP2K2 using rabbit polyclonal MAP2K2-S222 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).
Application

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.