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MEK 2 (phospho Thr394) Antibody

MEK 2 (phospho Thr394) Antibody MEK 2 (phospho Thr394) Antibody

Instruction Manual!

Product name: MEK 2 (phospho Thr394) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IFIHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

MEK 2 (phospho Thr394) Antibody

Catalog Number:ic284530

Product Profile

Product Name MEK 2 (phospho Thr394) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.1) Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221. Cowley S, et al. (1994) Cell. 77:841-852.
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Immunogen
MEK-2 (Phospho-Thr394) antibody was raised against a peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol MAP2K2
Alternative Names
CFC4
MEK2
MKK2
MAPKK2
PRKMK2
SRC1
ERK activator kinase 2
MAP kinase kinase 2
MAP2K2
MAPK/ERK kinase 2
MAPKK 2
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MolecularWeight(MW)  44 kDa
Tissue Specificity This antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.

Database Links

Entrez Gene 5605
Protein Accession NP_109587.1

Application

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).

  • Application

    Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).

ApplicationNotes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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