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GAPDH antibody - middle region
Instruction Manual!
| Product name: | GAPDH antibody - middle region |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-GAPDH polyclonal antibody - middle region
Catalog Number：IC117698
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Product Profile
| ProductName | Rabbit anti-GAPDH polyclonal antibody - middle region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-GAPDH antibody: synthetic peptide directed towards the middle region of human GAPDH |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | GAPDH |
|---|---|
| Gene Synonyms |
G3PD
More
GAPD MGC88685 |
| Gene Full Name | Glyceraldehyde-3-phosphate dehydrogenase |
| Gene Summary |
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a
More
|
| Alternative Names |
G3PD
More
GAPD MGC88685 |
| MolecularWeight(MW) | 36kDa |
| Sequence | 335 amino acids |
Database Links
| Entrez Gene | 2597 |
|---|---|
| SwissProt ID | P04406 |
| Protein Accession | NP_002037 |
Application
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Western blot
How many ug’s of tissue/cell lysate run on the gel: 20 ugWhat species+tissue/cell type run on the gel: HEK293Primary antibody dilution: 1 to 1000Secondary antibody: IRD800CW (anti-rabbit) (LICOR)Secondary antibody dilution: 1 to 20000
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Western blot
0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human kidney -
Immunoblot
1) 10 ug
2) 20 ug
3) 40 ug U87 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
HRP-goat anti-rabbit IgG(H+L) antibody
Secondary Antibody Dilution:
1: 15000
Gene Name:
GAPDH
Submitted by:
Prof. Varda Shushan-Barmatz, Ben-Gurion University
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
