VASP Antibody

Instruction Manual!

Product name:	VASP Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	IHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC294834

Product Name	VASP Antibody
Antibody Type	Primary Antibodies
Productdescription	Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.1) Barragan, P., et al. Thromb. Haemost. 104(2):410-411(2010) More
Immunogen	This VASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human VASP.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IHC-PWB
	For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	VASP
Alternative Names	Vasodilator-stimulated phosphoprotein VASP VASP More
Molecular Weight(MW)	40 kDa

Entrez Gene	7408
Protein Accession	P50552

Application

Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HUVEC whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Application

VASP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Application Notes	For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.