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CALR Antibody

CALR Antibody CALR Antibody

Instruction Manual!

Product name: CALR Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:ntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Applications:ELISAWB
Storage:For short periods of storage (days) store at 4˚C. For longer periods of storage, store CALR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
UOM:100ug

CALR Antibody

Catalog Number:IC273311

Product Profile

Product Name CALR Antibody
Antibody Type Primary Antibodies
Productdescription
							
Calreticulin is a multifunctional protein that acts as a major Ca (2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.1) Urade, R., et al., (2004) Biochemistry 43 (27), 8858-8868.
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Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CALR.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

ELISAWB

CALR antibody can be used for detection of CALR by ELISA at 1:312500. CALR antibody can be used for detection of CALR by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
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Species Reactivity

DogHumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CALR
Alternative Names
CALR
RO
CRT
SSA
cC1qR
HEL-S-99n
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Molecular Weight(MW) 48 kDa

Database Links

Entrez Gene 811
Protein Accession NP_004334

Application

  • Application

    Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.

Positive Control 1) Cat. No. 1211 - HepG2 Cell Lysate
ApplicationNotes
CALR antibody can be used for detection of CALR by ELISA at 1:312500. CALR antibody can be used for detection of CALR by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
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Additional Information

Form Liquid
StorageInstructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store CALR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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