PARP1 Antibody

Instruction Manual!

Product name:	PARP1 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC287383

Product Name	PARP1 Antibody
Antibody Type	Primary Antibodies
Productdescription	PARP1 is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.1) Tao,Z., Biochemistry 47 (21), 5804-5813 (2008) More
Immunogen	This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-214 amino acids from the N-terminal region of human PARP1.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFWB
	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	PARP1
Alternative Names	Poly [ADP-ribose] polymerase 1 PARP-1 ADP-ribosyltransferase diphtheria toxin-like 1 ARTD1 NAD(+) ADP-ribosyltransferase 1 ADPRT 1 Poly[ADP-ribose] synthase 1 PARP1 ADPRT PPOL More
Molecular Weight(MW)	113 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Mouse

Entrez Gene	142
Protein Accession	P09874

Application

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Western blot analysis in Hela cell line lysates (35ug/lane).
Application

Confocal immunofluorescent analysis of PARP1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application

Western blot analysis of PARP1 using rabbit polyclonal PARP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2).

ApplicationNotes	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.