NPM1 Antibody

Instruction Manual!

Product name:	NPM1 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC286472

Product Name	NPM1 Antibody
Antibody Type	Primary Antibodies
Productdescription	NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.1) Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009) Ma,W., Cancer Biomark(1), 51-58 (2009) Zhang,H., J. Biol. Chem. 279 (34), 35726-35734 (2004) More
Immunogen	This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human NPM1.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFIHC-PWB
	For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	NPM1
Alternative Names	Nucleophosmin NPM Nucleolar phosphoprotein B23 Nucleolar protein NO38 Numatrin NPM1 NPM More
Molecular Weight(MW)	33 kDa

Entrez Gene	4869
Protein Accession	P06748

Application

NPM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application

Western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).
Application

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Fluorescent image of A549 cell stained with NPM1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/

ApplicationNotes	For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.