APEX1 Antibody

Instruction Manual!

Product name:	APEX1 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	IFWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC271310

Product Name	APEX1 Antibody
Antibody Type	Primary Antibodies
Productdescription	Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells.1) Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009) More
Immunogen	This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human APEX1.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IFWB
	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	APEX1
Alternative Names	DNA-(apurinic or apyrimidinic site) lyase 31-- APEX nuclease APEN Apurinic-apyrimidinic endonuclease 1 AP endonuclease 1 APE-1 REF-1 Redox factor-1 DNA-(apurinic or apyrimidinic site) lyase mitochondrial APEX1 APE APE1 APEX APX HAP1 REF1 More
Molecular Weight(MW)	36 kDa

Entrez Gene	328
Protein Accession	P27695

Application

Confocal immunofluorescent analysis of APEX1 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application

Western blot analysis of anti-APEX1 Antibody in Hela cell line lysates (35ug/lane)
Application

Western blot analysis of APEX1 using rabbit polyclonal APEX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.

Application Notes	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.