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E2F1 antibody - middle region
Instruction Manual!
| Product name: | E2F1 antibody - middle region |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-E2F1 polyclonal antibody - middle region
Catalog Number：ic105269
Product Profile
| Product Name | Rabbit anti-E2F1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | E2F1 |
|---|---|
| Gene Synonyms |
E2F-1
More
RBAP1 RBBP3 RBP3 |
| Gene Full Name | E2F transcription factor 1 |
| Gene Summary |
The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
More
|
| Alternative Names |
E2F-1
More
RBAP1 RBBP3 RBP3 |
| MolecularWeight(MW) | 47kDa |
| Sequence | 437 amino acids |
Database Links
| Entrez Gene | 1869 |
|---|---|
| SwissProt ID | Q01094 |
| Protein Accession | NP_005216 |
Application
-
Western blot
Titration: 1.0 ug/ml
Positive Control: Fetal Lung -
Immunoblot
1. 30 ug Cytosol extract: Untransfected cells 2. 30 ug Cytosol extract: Cells transfected with control (non-targeting) siRNA 3. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA 4. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Goat anti-rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
E2F1
Submitted by:
Ewelina Swiderek, Insitute of Immunology and Experimental Therapy, Wroclaw, Poland
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
