MASTL Antibody (ascites)
Product name: | MASTL Antibody (ascites) |
Source: | Mouse |
Purity: | >95% |
Buffer Formulation: | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
Applications: | WB |
Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
UOM: | 100ug |
MASTL Antibody (ascites)
Catalog Number:IC213331
Product Profile
Product Name | MASTL Antibody (ascites) |
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Antibody Type | Primary Antibodies |
Key Feature
Clonality | Monoclonal |
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Isotype | IgG1 |
Clone Number | 235CT7.8.2.3 |
Host Species | Mouse |
Tested Applications | |
WB~~1:1000~16000:
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Species Reactivity | |
Concentration | 1mg/ml |
Purification |
Target Information
Gene Synonyms |
GW
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GWL THC2 |
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Alternative Names |
MASTL
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GW GWL THC2 Serine/threonine-protein kinase greatwall Microtubule-associated serine/threonine-protein kinase-like |
Moleculareight(MW) | 97319 Da |
Function |
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation
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Tissue Specificity | This MASTL monoclonal antibody is generated from mouse immunized with MASTL recombinant protein. |
Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Nucleus. Cleavage furrow. Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow |
Database Links
Entrez Gene | 84930 |
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Nucleotide Accession | NP_001165774.1;NP_001165775.1;NP_116233.2 |
Application
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Application
MASTL (Cat. #AM1911a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).
Application Notes |
WB~~1:1000~16000:
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Additional Information
Form | Liquid |
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StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.