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MASTL Antibody (ascites)

MASTL Antibody (ascites) MASTL Antibody (ascites)

Instruction Manual!

Product name: MASTL Antibody (ascites)
Source:Mouse
Purity:>95%
Buffer Formulation:Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Applications:WB
Storage:For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:100ug

MASTL Antibody (ascites)

Catalog Number:IC213331

Product Profile

Product Name MASTL Antibody (ascites)
Antibody Type Primary Antibodies

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 235CT7.8.2.3
Host Species Mouse
Tested Applications

WB

WB~~1:1000~16000:
Species Reactivity

Human

Concentration 1mg/ml
Purification

Target Information

Gene Synonyms
GW
GWL
THC2
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Alternative Names
MASTL
GW
GWL
THC2
Serine/threonine-protein kinase greatwall
Microtubule-associated serine/threonine-protein kinase-like
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Moleculareight(MW)  97319 Da
Function
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation
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Tissue Specificity This MASTL monoclonal antibody is generated from mouse immunized with MASTL recombinant protein.
Cellular Localization Cytoplasm, cytoskeleton, centrosome. Nucleus. Cleavage furrow. Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow

Database Links

Entrez Gene 84930
Nucleotide Accession NP_001165774.1;NP_001165775.1;NP_116233.2

Application

  • Application

    MASTL (Cat. #AM1911a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow).

Application Notes
WB~~1:1000~16000:

Additional Information

Form Liquid
StorageInstructions  For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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