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MAPK1 Antibody
Instruction Manual!
| Product name: | MAPK1 Antibody |
| Source: | Mouse |
| Purity: | >95% |
| Buffer Formulation: | Purified recombinant fragment of human MAPK1 expressed in E. Coli. |
| Applications: | WBFC |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
MAPK1 Antibody
Catalog Number：IC213192
Product Profile
| Product Name | MAPK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 3F8 |
| Host Species | Mouse |
| Tested Applications | |
|
WB~~1:500 - 2000. FC~~1:200 - 400.:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| Gene Synonyms |
ERK2
More
PRKM1 PRKM2 |
|---|---|
| Alternative Names |
ERK
More
p38 p40 p41 ERK2 ERT1 MAPK2 PRKM1 PRKM2 P42MAPK p41mapk MAPK1 |
| MolecularWeight(MW) | 41390 Da |
| Function |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. May play a role in the spindle assembly checkpoint
More
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| Tissue Specificity | Purified recombinant fragment of human MAPK1 expressed in E. Coli. |
| Cellular Localization | Cytoplasm, cytoskeleton, spindle (By similarity). Nucleus. Cytoplasm, cytoskeleton, centrosome Cytoplasm. Note=Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization |
Database Links
| Entrez Gene | 5594 |
|---|---|
| Nucleotide Accession | NP_620407.1 |
Application
-
Application
Figure 1: Western blot analysis using p44/42 MAPK mouse mAb against Jurkat (1), Hela (2), A431 (3) and NIH/3T3 (4) cell lysate.
-
Application
Figure 2: Flow cytometric analysis of Jurkat cells using p44/42 MAPK mAb (green) and negative control (purple).
| Application Notes |
WB~~1:500 - 2000. FC~~1:200 - 400.:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human MAPK1 expressed in E. Coli. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
