MMP2 Antibody

Instruction Manual!

Product name:	MMP2 Antibody
Source:	Mouse
Purity:	>95%
Buffer Formulation:	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Applications:	WBIFIHC
Storage:	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:	100ug

Catalog Number：IC213887

Product Name	MMP2 Antibody
Antibody Type	Primary Antibodies

Gene Synonyms	CLG4A
Alternative Names	MMP2 CLG4A 72 kDa type IV collagenase 72 kDa gelatinase Gelatinase A Matrix metalloproteinase-2 TBE-1 PEX More
MolecularWeight(MW)	73882 Da
Function	Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-\|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro- inflammatory NF-kappaB, NFAT and IRF transcriptional pathways More
Tissue Specificity	Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate
Cellular Localization	Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes

Entrez Gene	4313

Application

MMP2 western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MMP2 antibody detected the MMP2 protein (arrow).
Application

Confocal immunofluorescent analysis of MMP2 Antibody (Cat#AM1844b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Application

MMP2 Antibody (Cat. #AM1844b)immunohistochemistry analysis in formalin fixed and paraffin embedded human gastric carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application

MMP2 Antibody (Cat. #AM1844b)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Application Notes	WB~~1:100~250 IF~~1:10~50 IHC~~1:10~50:

Form	Liquid
StorageInstructions	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.