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MSH6 Antibody
Instruction Manual!
| Product name: | MSH6 Antibody |
| Source: | Mouse |
| Purity: | >95% |
| Buffer Formulation: | Purified recombinant fragment of MSH6 expressed in E. Coli. |
| Applications: | WB |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
MSH6 Antibody
Catalog Number：IC214665
Product Profile
| Product Name | MSH6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3A10H7 |
| Host Species | Mouse |
| Tested Applications | |
|
WB~~1:500 - 2000.:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| Gene Synonyms |
GTBP
|
|---|---|
| Alternative Names |
GTBP
More
HSAP HNPCC5 |
| MolecularWeight(MW) | 152786 Da |
| Function |
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair
More
|
| Tissue Specificity | Purified recombinant fragment of MSH6 expressed in E. Coli. |
| Cellular Localization | Nucleus. |
Database Links
| Entrez Gene | 2956 |
|---|---|
| Nucleotide Accession | NP_000170.1 |
Application
-
Application
Figure 1: Western blot analysis using anti-MSH6 monoclonal antibody against truncated MSH6 recombinant protein.
| Application Notes |
WB~~1:500 - 2000.:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of MSH6 expressed in E. Coli. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
