| ELISA kit |
| Clinical diagnosis |
| Trfia |
| ANTIBODIES |
| CLIA kits |
| Proteins |
| New Products |
| > Human |
| > Canine |
| > Bovine |
| > Equine |
| > Fish |
| > Mouse |
| > Rat |
| > Chicken |
| > Porcine |
| > Rabbit |
| > Goat |
| > Simian |
| > General |
| > Cavia |
| > Caprine |
| > Sheep |
| > Cattle |
Nephrin Antibody (Ascites)
Instruction Manual!
| Product name: | Nephrin Antibody (Ascites) |
| Source: | Mouse |
| Purity: | >95% |
| Buffer Formulation: | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Applications: | WBIF |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
Nephrin Antibody (Ascites)
Catalog Number：IC215204
Product Profile
| Product Name | Nephrin Antibody (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 174CT2.1.1 |
| Host Species | Mouse |
| Tested Applications | |
|
WB~~1:100~200 IF~~1:10~50:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| Gene Synonyms |
NPHN
|
|---|---|
| Alternative Names |
NPHS1
More
NPHN Nephrin Renal glomerulus-specific cell adhesion receptor |
| MolecularWeight(MW) | 134742 Da |
| Function |
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity)
More
|
| Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane |
Database Links
| Entrez Gene | 4868 |
|---|---|
| Nucleotide Accession | NP_004637.1 |
Application
-
Application
Nephrin Antibody (C-term) (Cat. #AM1865a) western blot analysis in SK-BR-3 cell line lysates (15ug/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).(1:100)
-
Application
Confocal immunofluorescent analysis of Nephrin Antibody (C-term) (Cat#AM1865a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
| Application Notes |
WB~~1:100~200 IF~~1:10~50:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
