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NPM1 Antibody
Instruction Manual!
| Product name: | NPM1 Antibody |
| Source: | Mouse |
| Purity: | >95% |
| Buffer Formulation: | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Applications: | WB |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
NPM1 Antibody
Catalog Number：IC215567
Product Profile
| Product Name | NPM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1042CT5.2.1 |
| Host Species | Mouse |
| Tested Applications | |
|
WB~~1:1000:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| Gene Synonyms |
NPM
|
|---|---|
| Alternative Names |
NPM1
More
NPM Nucleophosmin Nucleophosmin Nucleolar phosphoprotein B23 Nucleophosmin Nucleolar protein NO38 Nucleophosmin Numatrin |
| MolecularWeight(MW) | 32575 Da |
| Function |
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication
More
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| Tissue Specificity | Purified His-tagged NPM1 protein was used to produced this monoclonal antibody. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, centrosome. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis |
Database Links
| Entrez Gene | 4869 |
|---|
Application
-
Application
NPM1 Antibody (Cat. #AM2201b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).
| Application Notes |
WB~~1:1000:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
