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SMAD5 antibody - middle region
Instruction Manual!
| Product name: | SMAD5 antibody - middle region |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-SMAD5 polyclonal antibody - middle region
Catalog Number：IC105594
Product Profile
| ProductName | Rabbit anti-SMAD5 polyclonal antibody - middle region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-SMAD5 antibody: synthetic peptide directed towards the middle region of human SMAD5 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | SMAD5 |
|---|---|
| Gene Synonyms |
DWFC
More
JV5-1 MADH5 |
| Gene Full Name | SMAD family member 5 |
| Gene Summary |
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.
More
|
| Alternative Names |
DWFC
More
JV5-1 MADH5 |
| MolecularWeight(MW) | 51kDa |
| Sequence | 465 amino acids |
Database Links
| Entrez Gene | 4090 |
|---|---|
| SwissProt ID | Q99717 |
| Protein Accession | NP_005894 |
Application
-
Immunoblot
Lane 1: 5ug of transfected 293T lysate (SMAD1)
Lane 1: 5ug of transfected 293T lysate (SMAD2)
Lane 1: 5ug of transfected 293T lysate (SMAD3)
Lane 1: 5ug of transfected 293T lysate (SMAD4)
Lane 1: 5ug of transfected 293T lysate (SMAD5)
Lane 1: 5ug of transfected 293T lysate (SMAD6)
Lane 1: 5ug of transfected 293T lysate (SMAD7)
Lane 1: 5ug of transfected 293T lysate (SMAD8)
Lane 1: 5ug of transfected 293T lysate (GFP)
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit IgG HRP Conjugated
Secondary Antibody Dilution:
1:10,000
Gene Name:
SMAD5
Submitted by:
Amanda Urick, Medical College of Wisconsin -
Western blot
0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
