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GLI2 antibody - N-terminal region
Instruction Manual!
| Product name: | GLI2 antibody - N-terminal region |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WBIHC |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-GLI2 polyclonal antibody - N-terminal region
Catalog Number：IC105497
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Product Profile
| ProductName | Rabbit anti-GLI2 polyclonal antibody - N-terminal region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-GLI2 antibody: synthetic peptide directed towards the N terminal of human GLI2 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Protein A |
Target Information
| Gene Symbol | GLI2 |
|---|---|
| Gene Synonyms |
HPE9
More
THP1 THP2 |
| Gene Full Name | GLI family zinc finger 2 |
| Gene Summary |
GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
More
|
| Alternative Names |
HPE9
More
THP1 THP2 |
| MolecularWeight(MW) | 133kDa |
| Sequence | 1258 amino acids |
Database Links
| Entrez Gene | 2736 |
|---|---|
| SwissProt ID | P10070 |
| Protein Accession | NP_084655 |
Application
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Immunohistochemical
Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop
Primary Antibody Dilution :
1:50
Secondary Antibody :
Goat anti-rabbit-cy3
Secondary Antibody Dilution :
1:1500
Color/Signal Descriptions :
GLI2: Green Neural crest cells:: Red
Gene Name :
GLI2
Submitted by :
Anonymous -
Western blot
Host: Rabbit
Target Name:GLI2
Sample Tissue:Jurkat
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration:2.5ug/mL
Peptide Concentration: 4.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 6%-18% -
Western blot
Host: Rabbit
Target Name: GLI2
Sample Tissue: HepG2 Whole Cell lysates
Antibody Dilution: 1.0ug/ml
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
