Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)

Instruction Manual!

Product name:	Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)
Source:	Prokaryotic expression
Purity:	> 95%
Buffer Formulation:	PBS, pH7.4, containing 0.01% SKL.
Applications:	Positive Control; Immunogen; SDS-PAGE; WB.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
UOM:	100ug/50ug/200ug/1mg/1g

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residuess: Arg575~Asn826

Tags: N-terminal His Tag

Tissue Specificity: Secreted, Cytoplasm, Chromosome

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 0.01% SKL.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 28.7kDa

Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows: 1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved

to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure. Gene Sequencing (Extract)