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Recombinant Fibrinogen Beta (FGb)
Instruction Manual!
| Product name: | Recombinant Fibrinogen Beta (FGb) |
| Source: | N-terminal His-Tag |
| Purity: | >90% |
| Buffer Formulation: | Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative. |
| Applications: | SDS-PAGE; WB; ELISA; IP. |
| Storage: | Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. |
| UOM: | 10µg/50µg/100µg/200µg/1mg/1g |
[ PROPERTIES ]
Residues: Gly45~Gln491
Tags: N-terminal His-Tag
Accession: P02675
Host: E. coli
Subcellular Location: Secreted.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
Predicted isoelectric point: 7.6
Predicted Molecular Mass: 52.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
GHRPLD KKREEAPSLR PAPPPISGGG YRARPAKAAA TQKKVERKAP DAGGCLHADP
DLGVLCPTGC QLQEALLQQE RPIRNSVDEL NNNVEAVSQT SSSSFQYMYL LKDLWQKRQK
QVKDNENVVN EYSSELEKHQ LYIDETVNSN IPTNLRVLRS ILENLRSKIQ KLESDVSAQM
EYCRTPCTVS CNIPVVSGKE CEEIIRKGGE TSEMYLIQPD SSVKPYRVYC DMNTENGGWT
VIQNRQDGSV DFGRKW DPYK QGF GNVATNT D GKN YCGLPG E YW LGNDKIS
QLTRMGPTEL LIEMEDWKGD KVKAHYGGFT VQNEANKYQI SVNKYRGTAG NALMDGASQL
MGENRTMTIH NGMFFSTYDR DNDGW LTSDP RKQCSKEDGG GWW YNRCHAA
NPNGRYYWGG QYTWDMAKHG TDDGVVWMNW KGSWYSMRKM SMKIRPFFPQ Q
[ REFERENCES ]
1. Chung D.W., et al. (1983) Biochemistry 22:3244-3250.
2. Chung D.W., et al. (1990) Adv. Exp. Med. Biol. 281:39-48.
3. Huber P., et al. (1987) Nucleic Acids Res. 15:1615-1625.
4. Watt K.W.K., et al. (1979) Biochemistry 18:68-76.
