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Recombinant Estrogen Receptor Alpha (ERa)
Instruction Manual!
| Product name: | Recombinant Estrogen Receptor Alpha (ERa) |
| Source: | E. coli |
| Purity: | >99% |
| Buffer Formulation: | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. |
| Applications: | SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling. |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. |
| UOM: | 50µg/100µg/200µg/1mg/1g |
[ PROPERTIES ]
Source: Prokaryotic expression.
Host: E. coli
Residues: Ser178~Met438
Tags: N-terminal His-Tag
Homology: Mouse 89%, rat 88%
Tissue Specificity: Placenta, pituitary gland, mammary gland.
Subcellular Location: Nucleus.Cytoplasm.Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Purity: >99%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.0
Predicted Molecular Mass: 33.4kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. Gene Sequencing (Extract)
