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Recombinant Estrogen Receptor Alpha (ERa)
Instruction Manual!
| Product name: | Recombinant Estrogen Receptor Alpha (ERa) |
| Source: | E. coli |
| Purity: | >95% |
| Buffer Formulation: | <1.0EU per 1μg (determined by the LAL method). Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
| Applications: | SDS-PAGE; WB; ELISA; IP. |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. |
| UOM: | 50µg/100µg/200µg/1mg/1g |
[ PROPERTIES ]
Residues: Arg248~Leu484 (Accession # P06211), with two N-terminal Tags, His-tag and GST-tag.
Host: E. coli
Subcellular Location: Nucleus. Cytoplasm.
Golgi apparatus. Cell membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.6
Predicted Molecular Mass: 59.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-RKC YEVGMMKGGI
R K D R R G G R M L KH K R Q R D D L E G R N E M G T S G D MR A A N LW P S P LV I K H T K K N S
PALSLTADQM VSALLDAEPP LIYSEYDPSR PFSEASMMGL LTNLADRELV HMINWAKRVP
GFGDLNLHDQ VHLLECAWLE ILMIGLVWRS MEHPGKLLFA PNLLLDRNQG KCVEGMVEIF
DMLLATSSRF RMMNLQGEEF VCLKSIILLN SGVYTFLSST LKSLEEKDHI HRVL
