Recombinant Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Glu30~Pro322

Tags: N-terminal His Tag

Subcellular Location: n/a

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 100mMNaHCO₃, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%

Trehalose.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.6

Predicted Molecular Mass: 36.0kDa

Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.