Recombinant Keratin 1 (KRT1)

Instruction Manual!

Product name:	Recombinant Keratin 1 (KRT1)
Source:	E. coli
Purity:	>90%
Buffer Formulation:	Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Applications:	SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months.
UOM:	50µg/100µg/20µg/1mg/5mg

[ PROPERTIES ]

Residues: Ile350~Glu488

Tags: Two N-terminal Tags, His-tag and S-tag

Accession: Q6IMF3

Host: E. coli

Subcellular Location: Cell membrane.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 21.8kDa

Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

I AEVKAQYDSI CQRSKAEAET FYQSKYEELQ ITAGKHGDSV KNTKMEISEL NRVIQRLRSE

IDSVKKQISQ MQQNISDAEQ RGEKALKDAQ NKLNEIEDAL TQAKEELTRL LRDYQELMNT

KLALDMEIAT YRKLLEGE