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Recombinant Fibroblast Growth Factor 2, Basic (FGF2)
Instruction Manual!
| Product name: | Recombinant Fibroblast Growth Factor 2, Basic (FGF2) |
| Source: | E. coli |
| Purity: | >95% |
| Buffer Formulation: | 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose 0.01% sarcosyl and Proclin300. |
| Applications: | Positive Control; Immunogen; SDS-PAGE; WB |
| Storage: | Avoid repeated freeze/thaw cycles. |
| UOM: | 10µg/50µg/200µg/1mg/5mg |
[ PROPERTIES ]
Source: Prokaryotic expression.
Host: E. coli
Residues: Pro10~Ser154
Tags: N-terminal His-Tag
Subcellular Location: Secreted. Nucleus.
Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose 0.01% sarcosyl and Proclin300.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.6
Predicted Molecular Mass: 20.0kDa
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
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[ IDENTIFICATION ]
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Figure 1. SDS-PAGE
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
