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Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)

Polyclonal Antibody to Corticotropin Releasing Hormone (CRH) Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)

Instruction Manual!

Product name: Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
Source:
Purity:
Buffer Formulation:
Applications:WB, ICC, IHC-P, IHC-F, ELISA
Storage:
UOM:50µg/100µg/20µg/1mg/5mg

[ PRODUCT INFORMATION ]

Immunogen: CRH, Mouse Clonality: Polyclonal Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200µg/mL

UOM: 100µg


[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant CRH (Arg28~Arg160) with N-terminal His-Tag expressed in E.coli.

Accession No.: RPA835Mu01

 

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against CRH. It has been selected for its ability to recognize CRH in immunohistochemical staining and western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant CRH (Arg28~Arg160) disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5u L per well when used in enhanced chemilumescent (ECL).

Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

[ IMAGES ]


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