Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
Product name: | Polyclonal Antibody to Corticotropin Releasing Hormone (CRH) |
Source: | |
Purity: | |
Buffer Formulation: | |
Applications: | WB, ICC, IHC-P, IHC-F, ELISA |
Storage: | |
UOM: | 50µg/100µg/20µg/1mg/5mg |
[ PRODUCT INFORMATION ]
Immunogen: CRH, Mouse Clonality: Polyclonal Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CRH (Arg28~Arg160) with N-terminal His-Tag expressed in E.coli.
Accession No.: RPA835Mu01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against CRH. It has been selected for its ability to recognize CRH in immunohistochemical staining and western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant CRH (Arg28~Arg160) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5u L per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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